From The Grindhouse Cinema Database
- The first man who is re-animated at the morgue (who goes on to kill the dean) is Arnold Schwarzenegger's body double.
- Very loosely adapted from H.P. Lovecraft's "Herbert West - Re-Animator".
- Stuart Gordon and Dennis Paoli originally intended to be faithful to H.P. Lovecraft's story, but the film ultimately has little in common with the story, which was intended to be a parody of "Frankenstein".
- The special effects department went through 25 gallons of fake blood during the shoot.
- The "brains" in the severed head were made up of steer meat by-products, ground beef and fake blood and when they shot the scene in the autopsy room with the severed head being thrown out the door and then smashing onto the hallway wall, the crew were all behind the cameras with garbage bags over their clothes because no one knew just how much the brains would splatter.
- A poster for the Talking Heads documentary Stop Making Sense is visible above Dan's bed.
- The bald, bearded doctor at the foot of Megan's bed who gets shoved away as Dan tries to revive her is underground cartoonist Kim Deitch ("The Boulevard of Broken Dreams"), son of legendary Jazz-era cartoonist Gene Deitch ("The Cat").
- David Gale was made to shave his head and wear a toupee, as this was found to be in keeping with Dr. Hill's character. In the DVD commentary, it was revealed that this was also necessary for budgetary reasons, as there was no money available to match Gale's hair on a prosthetic head prop.
- The opening theme borrows heavily from the Alfred Hitchcock's Psycho opening theme by Bernard Herrmann.
- Richard Band went over schedule by two days while composing the score in Rome, Italy. As a direct result of this, Band had to invest $1,500 dollars of his own money in order to finish the score.
- Originally director Stuart Gordon wanted to shoot the movie in black and white on 16mm film to give the film a gritty quality.